Preferences ...
from the Edit menu:
This brings up the ROI Preferences dialog:

, the the default folder to save your ROIs will be the same as
the folder containing the image you currently have loaded. If you un-select this check-box, the
folder for saving ROIs will remain unchanged when you load a new image.
Press the
button to
set the ROI colours for this session only. Changes will not be
saved to your user preferences if you only press the
button.
To revert to the default setting press the
button. To save the settings to your
user preferences so that they will always be set when you start
Jim, press the
button.
Click the
button when
you have finished setting your ROI preferences.